All information updated in 2014

Publication – Schleiff, Bohnsack

The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana.

Weis BL, Missbach S, Marzi J, Bohnsack MT, Schleiff E. Plant J. 2014 Dec. Abstract Read more …

Publication – Wachtveitl, Suess

Tetracycline Determines the Conformation of Its Aptamer at Physiological Magnesium Concentrations.

Reuss AJ, Vogel M, Weigand JE, Suess B, Wachtveitl J. Biophys J. 2014 Dec 16. Abstract Read more …

04 Nov – Talk by Mario Mörl

As a joint seminar with the Institute for Molecular Biology, Prof. Mario Mörl, Universität Leipzig, gives a talk titled “Synthetic Riboswitches – Playing Lego with RNA Domains“.

Tuesday, 04/11/14 at 5:15 pm
Campus Riedberg, Biozentrum N260/3.13


Riboswitches are RNA-based regulators of gene expression composed of a ligand-sensing aptamer domain followed by an overlapping expression platform. This regulation occurs at either the level of transcription (by formation of terminator or antiterminator structures) or translation (by presentation or sequestering of the ribosomal binding site). Due to a modular composition, these elements can be manipulated and therefore represent useful tools to regulate gene expression in synthetic biology. While riboswitches for translational control have been designed successfully, attempts to construct synthetic elements regulating transcription have failed so far. Using the theophylline-aptamer as sensor, we designed the expression platform as RNA sequences that can fold into functional intrinsic terminator structures. Optimization of these terminator elements enhance the response ratio of the transcriptional riboswitches to the presence of a specific ligand. Furthermore, combination of several copies of individual riboswitches leads to a much improved activation ratio and to a linear dose-dependent response in reporter gene expression. Such synthetic riboswitches (as single elements or in serial arrangements) represent new regulatory elements for synthetic biology and can be used as efficient and fast-responding read-out systems.

25 June – Talk by Bettina Keller

Bettina KellerProf. Bettina Keller, FU Berlin, gives a talk titled “Complex RNA Folding Kinetics Revealed by Single-Molecule FRET and Hidden Markov Models”.

Wednesday, 25/06/14 at 5 pm

Seminar room N260/3.13, Biocenter, Campus Riedberg

11 Jun – Talk by Ruth Schmitz-Streit

Schmitz-StreitProf. Ruth Schmitz-Streit, Univ. of Kiel, gives a talk titled “Non-coding RNAs in Methanosarcina mazei are involved in regulation and defense”.

Wednesday, 11/06/14 at 5 pm

Seminar room N100/0.15, Biocenter, Campus Riedberg

2 June – Talk by Hervé Isambert

isambertHervé Isambert, Institut Curie, Paris, gives a talk titled “RNA switches and nanostructures under transcriptional control”.

Monday, 2/06/14 at 5:15 pm

Lecture hall N100/B3, Biocenter, Campus Riedberg

28 May – Talk by Cynthia Sharma

Cynthia SharmaCynthia Sharma, University of Würzburg, Research Center for Infectious Diseases (ZINF), gives a talk titled “Regulatory RNAs in the pathogenic Epsilonproteobacteria, Helicobacter pylori and Campylobacter jejuni“.

Wednesday, 28/05/14 at 5 pm
Seminar room N100/0.15, Biocenter, Campus Riedberg

Helicobacter pylori, the causative agent of gastritis, ulcers, and gastric cancer, and Campylobacter jejuni, the most common cause of bacterial gastroenteritis to date, are two major human pathogens. Genome sequencing revealed their potential proteins and genetic diversity, but less is known about the transcriptional organization and post-transcriptional regulation of these Gram-negative Epsilonproteobacteria. Previously, Helicobacter pylori was even regarded as an organism without riboregulation since it lacks, like 50% of all bacteria, the RNA chaperone Hfq. However, based on a novel differential RNA-sequencing approach (dRNA-seq), we had recently defined a genome-wide map of transcriptional start sites (TSS) and identified more than 60 sRNAs in H. pylori strain 26695. We have now applied a comparative dRNA-seq approach to multiple strains of the emerging food-borne pathogen, Campylobacter jejuni, to understand how transcriptome differences could contribute to phenotypic differences among closely related strains. Our study revealed that the majority of TSS is conserved among strains, but we also observed strain-specific promoter usage and small RNA (sRNA) repertoires, which could contribute to strain-specific gene regulation.

Based on our transcriptome datasets, we are now using Helicobacter and Campylobacter as new model organisms for riboregulation in virulent bacteria and bacteria that lack Hfq. We have started with the functional characterization of abundant sRNAs as well as identification of associated RNA-binding proteins and new regulatory mechanisms. For example, we could show that a trans-acting sRNA, RepG, from Helicobacter directly base-pairs with a homopolymeric G-repeat in the mRNA leader of the TlpB chemotaxis receptor and that length variation of the G-repeat determines the outcome (activation or repression) of RepG-mediated post-transcriptional regulation. Overall, the identification and characterization of diverse sRNA candidates in Helicobacter and Campylobacter indicates that riboregulation constitutes an important layer of gene-expression control in these major human pathogens and will provide new insights into regulatory mechanisms and virulence control.

Publication – Tampé, Wachtveitl, Heckel

Three-dimensional protein networks assembled by two-photon activation.

Gatterdam V, Ramadass R, Stoess T, Fichte MA, Wachtveitl J, Heckel A, Tampé R. Angew Chem Int Ed Engl. 2014 May 26. Abstract Read more …

Publication – Wachtveitl

Photo-physical properties of 2-(1-ethynylpyrene)-adenosine: Influence of hydrogen bonding on excited state properties.

Trojanowski P, Plötner J, Grünewald C, Graupner F, Slavov C, Reuss A, Braun M, Engels JW, Wachtveitl J. Phys Chem Chem Phys, 2014 May 14. Abstract Read more …

30 April – Talk by Sander Granneman

Bettina KellerSander Granneman, University of Edinburgh, Scotland, gives a talk titled “CRAC’n the dynamics of Ribosome assembly”.

Wednesday, 30/04/14 at 5 pm

Seminar room N100/0.15, Biocenter, Campus Riedberg

CANCELLED – Talk by Karola Rück-Braun

Prof. Karola Rück-Braun, TU Berlin, gives a talk titled “Photoswitches for the Life Sciences And Material Sciences”.

Unfortunately, the talk on Wednesday, 16/04/14 at 5 pm had to be CANCELLED.

Seminar room N100/0.15, Biocenter, Campus Riedberg

Publication – Wachtveitl, Schwalbe

Mechanism of the photoinduced uncaging reaction of puromycin protected by a 6-nitroveratryloxycarbonyl group.

Kohl-Landgraf J, Buhr F, Lefrancois D, Mewes JM, Schwalbe H, Dreuw A, Wachtveitl J. J Am Chem Soc, 2014 Mar 05. Abstract Read more …

Publication – Burghardt

Ultrafast C_spiro-O Dissociation via a Conical Intersection Drives Spiropyrane to Merocyanine Photoswitching.

Prager S, Burghardt I, Dreuw A. J Phys Chem A. 2014 Feb 27. Abstract Read more …


Publication – Schleiff, Bohnsack

The evolution of the ribosome biogenesis pathway from a yeast perspective.

Ebersberger I, Simm S, Leisegang MS, Schmitzberger P, Mirus O, von Haeseler A, Bohnsack MT, Schleiff E. Nucleic Acids Res. 2014 Feb 1. Abstract Read more …

Publication – Rother, Suess

Development of β-Lactamase as a Tool for Monitoring Conditional Gene Expression by a Tetracycline-Riboswitch in Methanosarcina acetivorans.

Demolli S, Geist M, Weigand J, Matschiavelli N, Suess B and Rother M. Archaea, 2014 Jan 20. Abstract Read more …

Publication – Schwalbe, Heckel

Influence of the absolute configuration of npe-caged cytosine on DNA single base pair stability.

Steinert HS, Schäfer F, Jonker HR, Heckel A, Schwalbe H. Angew Chem Int Ed Engl, 2014 Jan 20. Abstract Read more …

Publication – Schwalbe

RNA refolding studied by light-coupled NMR spectroscopy.

Schwalbe H, Fürtig B. Methods Mol Biol, 2014. Abstract Read more …