All information updated in December, 2019

Guest Speaker – Boris Görke

On Thursday, 12.12.2019, 5.15 pm

 

Boris Görke (Max-Perutz-Labs, Wien), will give an SFB 902 lecture:

„A regulatory network controlling bacterial cell envelope synthesis at the post-transcriptional level“.

Venue: TU Darmstadt, Campus Botanischer Garten, lecture hall: B101/52

Guests are welcome!
Abstract:
Gram-negative bacteria such as E. coli possess a cell envelope consisting of peptidoglycan and an outer membrane, which provides protection against environmental stresses. Synthesis of glucosamine-6-phosphate (GlcN6P) by enzyme GlmS represents the first and rate-limiting step in the cell envelope synthesis pathway. In order to ensure continuous cell envelope synthesis, GlcN6P homeostasis is required, which is achieved through a post-transcriptional regulatory circuit composed of small RNAs (sRNAs) GlmY and GlmZ and the RNA-binding protein RapZ. GlmZ activates glmS translation by base-pairing. When GlcN6P is ample, GlmZ is bound by adaptor protein RapZ and inactivated by cleavage through recruitment of RNase E. Decreasing GlcN6P concentrations provoke accumulation of the homologous sRNA GlmY, which acts as decoy and sequesters RapZ thereby counteracting GlmZ decay. The crystal structure of RapZ revealed a novel type of RNA-binding protein, which putatively evolved from re-purpose of a glycolytic enzyme. The active form of RapZ corresponds to a tetramer, which may activate the likewise tetrameric RNase E in an encounter complex to cleave sRNA GlmZ that is presumably sandwiched between the two tetramers. Notably, RapZ also represents the sought GlcN6P sensor: RapZ binds GlcN6P with high affinity and rapZ mutants are blind for this metabolite. Upon GlcN6P depletion, RapZ stimulates a transcription factor through interaction to increase GlmY expression levels. Thus, RapZ has a moonlighting function and controls production of its own decoy GlmY, which subsequently sequesters the available RapZ protein into long-lived ribonucleoprotein complexes, thereby counteracting GlmZ decay.

RNA Club — Wednesday — 11.12.2019 — 5 pm — Biozentrum — Room N100 0.15

Dear friends of RNA,

Our next RNA Club will take place on December, 11th at 5 pm.

We have an exciting program that you can find in the attached flyer
and under the usual links:

www.rnaclub.de or www.sfb902.de

We hope to see you there and discuss over Glühwein and Pizza.

Michaela & Beatrix

 

Program: RNACLUB_20191211

 


Michaela Müller-McNicoll (Ph.D.)

Juniorprofessor “RNA Regulation in Higher Eukaryotes”

Cluster of Excellence Macromolecular Complexes (CEF)
Goethe University Frankfurt
Institute for Cell Biology and Neuroscience
Max-von-Laue-Str. 13
60438 Frankfurt am Main

Tel: 069 798 42079
http://www.bio.uni-frankfurt.de/52678296/Group