‘Front Page Featured’

Guest Speaker – Boris Görke

On Thursday, 12.12.2019, 5.15 pm

 

Boris Görke (Max-Perutz-Labs, Wien), will give an SFB 902 lecture:

„A regulatory network controlling bacterial cell envelope synthesis at the post-transcriptional level“.

Venue: TU Darmstadt, Campus Botanischer Garten, lecture hall: B101/52

Guests are welcome!
Abstract:
Gram-negative bacteria such as E. coli possess a cell envelope consisting of peptidoglycan and an outer membrane, which provides protection against environmental stresses. Synthesis of glucosamine-6-phosphate (GlcN6P) by enzyme GlmS represents the first and rate-limiting step in the cell envelope synthesis pathway. In order to ensure continuous cell envelope synthesis, GlcN6P homeostasis is required, which is achieved through a post-transcriptional regulatory circuit composed of small RNAs (sRNAs) GlmY and GlmZ and the RNA-binding protein RapZ. GlmZ activates glmS translation by base-pairing. When GlcN6P is ample, GlmZ is bound by adaptor protein RapZ and inactivated by cleavage through recruitment of RNase E. Decreasing GlcN6P concentrations provoke accumulation of the homologous sRNA GlmY, which acts as decoy and sequesters RapZ thereby counteracting GlmZ decay. The crystal structure of RapZ revealed a novel type of RNA-binding protein, which putatively evolved from re-purpose of a glycolytic enzyme. The active form of RapZ corresponds to a tetramer, which may activate the likewise tetrameric RNase E in an encounter complex to cleave sRNA GlmZ that is presumably sandwiched between the two tetramers. Notably, RapZ also represents the sought GlcN6P sensor: RapZ binds GlcN6P with high affinity and rapZ mutants are blind for this metabolite. Upon GlcN6P depletion, RapZ stimulates a transcription factor through interaction to increase GlmY expression levels. Thus, RapZ has a moonlighting function and controls production of its own decoy GlmY, which subsequently sequesters the available RapZ protein into long-lived ribonucleoprotein complexes, thereby counteracting GlmZ decay.

Guest Speaker – Chase Beisel

On Thursday, 21.11.2019, 5.15 pm

 

Chase Beisel (Helmholtz Zentrum für Infektionsforschung, Würzburg), will give an SFB 902 lecture:

„From CRISPR biology to versatile technologies“.

Venue: TU Darmstadt, Campus Botanischer Garten, lecture hall: B101/52

Guests are welcome!

Guest Speaker – Marina Chekulaeva

On Tuesday, 05.11.2019, 5.15 pm

 

Marina Chekulaeva (Max-Delbrück-Zentrum, Berlin), will give an SFB 902 lecture:

„Mechanisms of RNA localization in neurons“.

Venue: Campus Riedberg, Lecture hall N260/3.13

Guests are welcome!
Abstract:
The proper subcellular localization of RNAs and proteins is crucial for their function. It is particularly important for highly polarized cells, such as oocytes, migrating andgrowing cells, neurons. In mRNAs, they are mediated by specific cis-regulatory elements, so called zip-codes. These elements are bound by trans-acting factors (RBPs, miRNAs), which regulate transport, stability or translation. So far, our knowledge is restricted to only a few examples of zip-codes and regulatory factors. We aim to identify these elements genome-wide and dissect molecular mechanisms underlying their function. To identify proteins and RNAs that are differentially localized and translated between neuronal subcellular compartments – neurites and soma – we developed a neurite/soma separation scheme in combination with mass spectrometry, RNA-seq, 3′-mRNAseq, Ribo-seq and bioinformatic analyses. Our results demonstrate that mRNA localization is the primary mechanism for protein localization in neurites and may account for more than a half of the neurite-localized proteome. Moreover, we identified multiple neurite-targeted non-coding RNAs and RBPs with potential regulatory roles. Using a combination of PAR-CLIP, RIP, and CRISPR/Cas-mediated knockouts, we are dissecting the roles of selected neurite-targeted RBPs and miRNAs in establishment of neuronal polarity. Relying on the same neurite/soma separation scheme, we are mapping cis-regulatory elements mediating RNA localization and investigating architecture of local proteome, transcriptome and translatome in motor neuron disorders.

Publication – Heilemann, Schuman

Super-resolution imaging and estimation of protein copy numbers at single synapses with DNA-point accumulation for imaging in nanoscale topography.

C. Böger, A.-S. Hafner, T. Schlichthärle, M. T. Strauss, S. Malkusch,U. Endesfelder, R. Jungmann, E. M. Schuman, M. Heilemann.
Solid State NMR

Full publication here: Super-resolution imaging and estimation of protein copy numbers at single synapses with DNA-point accumulation for imaging in nanoscale topography

Abstract:  Read more …

Summer School on Nucleic Acid Chemistry and Synthetic Biology

28th July – 2nd of August 2019, Würzburg, Germany:

The faculty of Chemistry and Pharmacy at the University of Würzburg (Germany) will organize an International Summer School on Nucleic Acid Chemistry and Synthetic Biology from 28th July – 2nd of August 2019 (http://go.uni-wuerzburg.de/summerschool).

The scientific program offers lectures and workshops on various topics in the field of nucleic acids research, with a focus on functional nucleic acids and synthetic as well as natural DNA and RNA modifications. The school is aimed at advanced Master’s as well as Ph.D. students or postdocs. You and your research group may be interested in the topics presented, so I wanted to inform you about the Summer School (see attached Poster).

We would be delighted to welcome your students to Würzburg. Students will also have the chance to present their research projects on posters, and some abstracts will be selected for short talks. The summer school is free of charge (excluding accommodation) and enrolled participants can earn a certificate for active participation in the summer school.

If you have any questions or need further information please feel free to contact us:

Summer School on Nucleic Acid Chemistry and Synthetic Biology
Faculty of Chemistry and Pharmacy
University of Wuerzburg
Am Hubland, D-97074 Wuerzburg
Fon: +49(0)931 31 81813
Fax: +49(0)931 31 84607
Mail: email hidden; JavaScript is required
Internet: go.uni-wuerzburg.de/summerschool

SummerSchool_2019_Nucleic_Acid_Chemistry

Aptamers in Bordeaux – June 28-29 2019 – International Conference

Conference Homepage

IRTG Summer School

The last Summer School of the SFB 902’s 2nd funding period will take place in Bayreuth from June 24th to June 28th.
We are looking forward to 4 days of scientific program, guest speaker Claus Dieter Kuhn and an exceptional free time adventure!
35 places available, first come first serve – register now!

Registration

Hausprospekt

Publication – Corzilius, Suess

Dynamic nuclear polarization on a hybridized hammerhead ribozyme: An explorative study of RNA folding and direct DNP with a paramagnetic metal ion cofactor.

D. Daube,  M. Vogel,   B. Suess,  B. Corzilius.
Solid State NMR

Full publication here: Dynamic nuclear polarization on a hybridized hammerhead ribozyme: An explorative study of RNA folding and direct DNP with a paramagnetic metal ion cofactor

Abstract:  Read more …

6th RNA Salon

The 6th RNA Salon will take place on April 8th, 2019 — 12:30 pm 

Venue: FIAS Campus Riedberg — Room 0.100

6. RMU-RNA Salon – Flyer(1)

Program:

Presenting groups:

Reinier Boon (ICR, GU Frankfurt) – lncRNAs & aging

Sven Danckwardt (CTH, JGU Mainz) – alternative polyadenylation

Christoph Dieterich (DZHK, Heidelberg) – computational RNA biology

Martin Hengesbach (IOCCB, GU Frankfurt) – dynamics of RNA modifications

Meike Saul (Biology, TU Darmstadt) – extracellular RNAs

Jennifer Winter (IHG, JGU Mainz) – miRNAs & neurobiology

We have also 4 Short talks from selected abstracts! The best talk will win a price sponsored by GeneXPro!

Please register until thursday, 4.4.2019 for free at http://bit.do/RMU-RNA-Salon6!

We cordially invite you to stay for diner and meet with the speakers.

Contact:

Michaela Müller-McNicoll (Ph.D.)

Juniorprofessor “RNA Regulation in Higher Eukaryotes”

Cluster of Excellence Macromolecular Complexes (CEF)
Goethe University Frankfurt
Institute for Cell Biology and Neuroscience
Max-von-Laue-Str. 13
60438 Frankfurt am Main

Tel: 069 798 42079
http://www.bio.uni-frankfurt.de/52678296/Group

SYNPEP – ERC Grant for Helge Bode

Press release Goethe University

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GRADE-Trainingsprogramm für das Sommersemester 2019 online

Sehr geehrte Damen und Herren,

liebe Programmkoordinatorinnen, liebe Programmkoordinatoren,

hiermit möchte ich Sie darüber informieren, dass das neue GRADE-Trainingsprogramm für das Sommersemester 2019 nun online ist.

Auch diesmal enthält das Programm eine Reihe bewährter Workshops sowie neue Themen, die wir in diesem Semester anbieten möchten. Zudem finden Sie in unserer Übersicht alle universitätsweite Angebote für Promovierende und Postdocs.

Alle Informationen zum aktuellen Angebot finden Sie auf unserer Website unter dem Link: http://tinygu.de/f766.

Die Broschüre zum GRADE-Trainingsprogramm kann online über folgenden Link abgerufen werden: http://www.uni-frankfurt.de/76660797/Brosch%C3%BCre_SoSe19_Online.pdf

Publication – Corzilius, Suess

Complex formation of the tetracycline‐binding aptamer investigated by specific cross‐relaxation under DNP.

V. Aladin,  M. Vogel,  R. Binder,  I. Burghardt,  B. Suess,  B. Corzilius.
Angewandte Chemie Int. Ed. 2019

Full publication here: Complex formation of the tetracycline‐binding aptamer investigated by specific cross‐relaxation under DNP

Abstract:  Read more …

RNA Club

Dear friends of RNA,

Save the date. The next RNA Club will take place on February, 27th at 5 pm.

Room: N260 / 313

The program will be announced very soon (www.rnaclub.de).

We hope to see you there and discuss over beer and Pizza.

Michaela & Beatrix

Guest Speaker – Janosch Hennig

On Thursday, 07.02.2019, 4.00 pm

 

Janosch Hennig (EMBL, Heidelberg), will give an SFB 902 lecture:

„Structural insights into MLE-Unr-roX2 complex assembly during early
steps of Drosophila dosage compensation“
.Venue: TU Darmstadt, Campus Botanischer Garten, lecture hall: B101/52

Guests are welcome!

Guest Speaker – Amanda E. Hargrove

On Thursday, 17.01.2018, 4.15 pm

 
Prof. Amanda E. Hargrove, Duke University Medical School Durham, North Carolina, USA, will give an SFB 902 lecture

„Deciphering patterns in selective small molecule: RNA interactions“.

Venue: Goethe University, Campus Riedberg, Lecture Hall N100/B3

Guests are welcome!

Guest Speaker – Andrea Sinz

On Tuesday, 27.11.2018, 4.15 pm

 
Prof. Dr. Andrea Sinz, Martin-Luther University Halle-Wittenberg, will give an SFB 902 lecture

Cross-Linking/Mass Spectrometry for Investigating Protein Complexes: Where Are We Now and Where Should We Go From Here?“.Venue: Goethe University, Campus Riedberg, seminar room N100/1.14

Guests are welcome!

Guest Speaker – Krzysztof Sobczak

On Thursday, 15.11.2018, 5.15 pm

 
Prof. Dr. Krzysztof Sobczak, Adam Mickiewicz University, Poznań / Poland, will give an SFB 902 lecture

Transcriptome changes caused by toxic RNA in myotonic dystrophy“.Venue: TU Darmstadt, Campus Botanischer Garten, lecture hall: B101/52

Guests are welcome!
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5. RMU-RNA SALON 2018

Dear friends of RNA,

we are happy to announce the 5. RMU-RNA Salon event “Genomics approaches in RNA Biology”.

5. RMU-RNA Salon – Flyer

The RMU-RNA Salon is designed as a series of mini-symposia to bring together scientists interested in RNA biology within the Rhine-Main area to increase the awareness for available methods and to fuel the interactions:

When: Wednesday, October 24th 2018, 12:30 am – 6:30 pm

Where: Biomedizinisches Forschungszentrum (BMFZ) Marburg

Keynote:

Katja Strässer (IBC, JLU Gießen) – mRNA packaging & export

Presenting groups:

Arnold Grünweller (IPC, PU Marburg) – RNA viruses

Aline Koch (IPP, JLU Gießen) – RNAi in plants

Julien Bethune (BZH, Uni Heidelberg) – RBPs & endomembranes

Andreas Krueger (IMM, GU Frankfurt) – miRNAs & T-cell development

Marcel Schulz (ICR, GU Frankfurt) – Systems Biology

Jennifer Winter (IHG, JGU Mainz) – miRNAs & neuronal development

Please register for free at http://bit.do/RMU-RNA-Salon5 !

We cordially invite you to present your own research and meet with the speakers.

Abstracts for short talks and posters are welcome!

Guest Speaker – Boris Eliseev

Next week – on Friday, 7th September 201812:30 h s.t.

 
Dr. Boris Eliseev, EMBL, Grenoble / France, will give a lecture
in a joint SFB 902 & SFB 807 seminar:

„Structure of a human cap-dependent 48S translation pre-initiation complex“.
Venue: Biocenter N100, Lecture Hall B3 / Campus Riedberg
Guests are welcome!

Understanding RNA-based Regulation in Cells -Registration NOW open!!

SFB 902 Symposium – 29.+30.08.2018

Otto-Stern-Zentrum, Campus Riedberg

Registration

more Info Read more …

Publication – Fürtig, Schwalbe

Conformational switch in the ribosomal protein S1 guides unfolding of structured RNAs for translation initiation.

N.S. Qureshi, S. Sreeramulu, J.K. Bains, H. Schwalbe*, B. Fürtig*

Nucleic Acids Res. in press

 

Publication – Wöhnert

Adenine protonation  enables c-di-GMP binding to cyclic-GAMP sensing riboswitches.

H. Keller, A. K. Weickhmann, T. Bock, J. Wöhnert.
RNA. 2018

Full publication here: Adenine protonation enables cyclic-di-GMP binding to cyclic-GAMP sensing riboswitches.

Abstract:  Read more …

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Publication – Wöhnert

An intricate balance of hydrogen bonding, ion atmosphere and
dynamics facilitates a seamless uracil to cytosine substitution in the
U-turn of the neomycin-sensing riboswitch.

M. Krepl, J. Vögele, H. Kruse, E. Duchardt-Ferner, J. Wöhnert, J.
Sponer.
Nucleic Acids Res. 2018

Full publication here: An intricate balance of hydrogen bonding, ion atmosphere and dynamics facilitates a seamless uracil to cytosine substitution in the U-turn of the neomycin-sensing riboswitch

Abstract:  Read more …

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Publication – Göringer

The OB-fold proteins of the Trypanosoma brucei editosome execute RNA-chaperone activity,

C. Voigt, M. Dobrychłop, E. Kruse, A. Czerwoniec, J. M. Kasprzak, P. Bytner, C. Del Campo, W-M. Leeder, J. M. Bujnicki, H. U. Göringer.
Nucleic Acids Research. 2018

Full publication here: The OB-fold proteins of the Trypanosoma brucei editosome execute RNA-chaperone activity

Abstract:  Read more …

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Publication – Heckel, Schwalbe

Chemo-enzymatic synthesis of position-specifically modified RNA for biophysical studies including light control and NMR spectroscopy.

Schwalbe H, Keyhani S, Goldau T, Blümler A, Heckel A.
Angew Chem Int Ed Engl. 2018

Full publication here: Chemo-enzymatic synthesis of position-specifically modified RNA for biophysical studies including light control and NMR spectroscopy.

Abstract:  Read more …

Publication – Heilemann

Correlative Single-Molecule FRET and DNA-PAINT Imaging.

Deußner-Helfmann NS, Auer A, Strauss MT, Malkusch S, Dietz MS, Barth HD, Jungmann R, Heilemann M.
Nano Lett. 2018

Full publication here: Correlative Single-Molecule FRET and DNA-PAINT Imaging

Abstract:  Read more …

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4. RMU-RNA SALON 2018

4. Mini-Symposium “Molecular mechanisms of posttranscriptional regulation” on methods to study processing, structure, and translation of mRNAs as well as non-coding RNAs in disease-related contexts.

Keynote Speaker:

  • Stefanie Dimmeler (ICR, GU Frankfurt) – non-coding RNAs

Guest speaker:

  • Veronika Herzog (IMBA, Vienna) – metabolic RNA-seq

more information available on the Homepage of the IMB, Mainz

Rolf Sammet Guestprofessorship 2018: Eric Westhof

RNA – Chemistry and Architecture
May 21—25 | 2018

 

more info: Sammet Homepage of the Goethe University

 

Guest Speaker – Konrad Förstner

Konrad Förstner, Head of the Core Unit Systems Medicine
Julius-Maximilians-Universität Würzburg will give a talk titled:

Translating high-throughput sequencing data into biological insights

Thursday, 03th May 2018
17.15 pm

Technische Universität Darmstadt

Campus Botanischer Garten, N101/lecture hall 52

Guest Speaker – Kwaku Dayie

Kwaku Dayie
University of Maryland

What is the big deal about ‘invisible’ RNA conformational states in Biology?

Thursday, 29th March 2018
4.00 pm

Goethe-University Frankfurt

Campus Riedberg, N100/lecture hall B2

Guest Speaker – Dr. Sabine Brantl

Dr. Sabine Brantl
AG Bakteriengenetik, Universität Jena

Small RNAs and small peptides

Tuesday, 27th March 2018
3.00 pm

Goethe-University Frankfurt

Campus Riedberg, N100/lecture hall B2

IRTG Winter School

This year’s Winter School will take place in Altglashütten, Schwarzwald, from March 4th to 8th.

Exciting talks, fun in the snow and  intensive interactions will hopefully lead to an enjoyable event and, eventually, to successful PhD projects

Please register here

EMBO course – iCLIP: Genomic views of protein-RNA interactions

Dear friends of RNA,

we are happy to announce the third edition of our EMBO course “iCLIP: Genomic views of protein-RNA interactions’, which will take place at the CRG in Barcelona from June 3rd to June 9th, 2018.

The course is targeted to Junior researchers (mainly PhDs and Postdocs) who are interested in Protein/RNA interactions.

We would be grateful  if you could forward this email to interested colleagues.

Registration deadline is March 1st 2018.

You can find more info about the course here

RNA Club

Dear friends of RNA,

The next RNA Club will take place on February, 28th at 5 pm.

Room and program will be announced soon (www.rnaclub.de).

We hope to see you there and discuss over beer and Pizza.

Michaela & Beatrix

contact for more info:
Michaela Müller-McNicoll (Ph.D.)
http://www.bio.uni-frankfurt.de/52678296/Group

Guest Speaker – Dr. Zasha Weinberg

Dr. Zasha Weinberg
Department of Computer Science, Leipzig University

Computational discovery of novel classes of bacterial non-coding RNAs

Thursday, 8th February 2018
12.30 pm
TU Darmstadt, lecture hall: B261/102

Abstract: The discovery of new classes of non-coding RNAs (ncRNAs) can reveal information about the biological processes that involve these ncRNAs as well as yielding general insights into RNA biochemistry and gene regulation. Riboswitch RNAs directly sense a metabolite and regulate genes accordingly. We devised a strategy to find riboswitches that have evolved specificity to a new metabolite ligand. Such specificity changes are well known in proteins, but only a few examples are known in RNA. I will also describe a more general search strategy for novel structured ncRNAs on a multi-genome/metagenome scale. This search revealed 224 novel ncRNAs of various types. I will also show computational predictions that have been experimentally confirmed.

 

Publication – Prisner, Sigurdsson

A semi-rigid isoindoline-derived nitroxide spin label for RNA.

Gophane DB, Endeward B, Prisner T F, and Sigurdsson ST.
Org. Biomol. Chem, 2018 Advance Article.

Full publication here: A semi-rigid isoindoline-derived nitroxide spin label for RNA

Abstract:  Read more …

Publication – Süß

Riboswitching with ciprofloxacin—development and characterization of a novel RNA regulator

Groher F, Bofill-Bosch C, Schneider C, raun J, Jager S, Geißler K, Hamacher K, Süß B.
Nucleic Acids Research, 2018 Jan 13.

Full publication here: Riboswitching with ciprofloxacin—development and characterization of a novel RNA regulator

Abstract:  Read more …

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Annual IRTG Symposium on RNA Biology

 

 

From Wednesday, Nov 15 to Thursday, Nov 16, the annual IRTG Symposium will take place in Friedrichsdorf/Taunus. Topics will cover all fields of RNA biology that are research areas within our CRC 902. The following Guest Speakers are presenting their research alongside members of our IRTG:

Nadine Schwierz-Neumann – MPI for Biophysics, Frankfurt
Niels Gehring – Universität zu Köln
Markus Wahl – Freie Universität Berlin
Renee Schröder – Max F. Perutz-Laboratories, Wien
Mario Mörl – Universität Leipzig
Jörg Hartig – Universität Konstanz
Christoph Dieterich – Uniklinikum Heidelberg
David Stanek – The Czech Academy for Sciences
Alfredo Castello – University of Oxford

Registration is now open via the following link.

Program:

Das Proteom aktiver Nervenzellen

Pressemitteilung

Das Proteom aktiver Nervenzellen

Max-Planck-Forscher entwickeln eine Technik, mit der sie neue Proteine in unterschiedlichen Zelltypen des Mäusegehirns messen können

Das Gehirn besteht aus einer Vielzahl unterschiedlicher Typen von Nerven- und Gliazellen. Da sie alle das gleiche Erbgut besitzen, muss diese Vielfalt auf den von ihnen gebildeten Proteinen beruhen. Für einen bestimmten Zelltyp konnte die Gesamtheit der Proteine – das sogenannte Proteom –bislang nicht bestimmt werden. Wissenschaftler der Max-Planck-Institute für Hirnforschung und für Biophysik in Frankfurt haben nun neue Methoden entwickelt, mit denen sie Änderungen im Proteom in einem bestimmten Zelltyp erkennen können. Sie veröffentlichen diese Ergebnisse und deren Bedeutung in der Zeitschrift Nature Biotechnology.

Read more …

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2nd RMU-RNA Salon

On Wednesday, June 28, the Goethe-Universität Frankfurt, in association with the SFB 902 and the RNA Society, will host the 2nd RNA Salon of the Rhein-Main Universitäten Associacion. Keynote Speaker will be Erin Schuman of the MPI for Brain Research, who recently received her second ERC advanced grant.

Presenting Groups:

AG Legewie (IMB Mainz) – Modelling of Biological Networks

AG Preußer (BCI, JLU Gießen) – CircRNAs & Exosomes

AG Randau (MPI Marburg) – Small RNomics & RIP-seq

AG Roignant (IMB Mainz) – RNA Modifications

AG Schmidt (IBC1, GU Frankfurt) – Ribo-seq

AG Zarnack (BMLS, GU Frankfurt) – Computational RNA Biology

Read more …

Publication – Schwalbe, Sigurdsson

Impact of spin label rigidity on extent and accuracy of distance information from PRE data.

Schnorr KA, Gophane DB, Helmling C, Cetiner E, Pasemann K, Fürtig B, Wacker A, Qureshi NS, Gränz M, Barthelmes D, Jonker HRA, Stirnal E, Sigurdsson ST, Schwalbe H.
Nucleic Acids Research, 2017 May 12.

Full publication here: Impact of spin label rigidity on extent and accuracy of distance information from PRE data.

Abstract:  Read more …

Guest Speaker – Anna Ephrussi

Dear friends of RNA,

We are pleased to announce the first speaker of the Minerva Lecture series:

Anne Ephrussi
European Molecular Biology Laboratory (EMBL)
Heidelberg

Assembly of mRNPs for transport within and between cells of the Drosophila germline

Wednesday, March 22, 2017

4:00 pm

Hosts:
Erin Schuman
MPI for Brain Research
Synaptic Plasticity

Michaela Müller-McNicoll
Cluster of Excellence Macromolecular Complexes (CEF)
Goethe Universität Frankfurt

Lecture hall, MPI for Brain Research
Max-von-Laue-Str. 4, 60438 Frankfurt