A10 – Björn Corzilius

RNA structure elucidation using site-specific dynamic nuclear polarization in the solid state

Site-specific dynamic nuclear polarization (DNP) on RNA is a potential method for extracting structural information using magic-angle spinning (MAS) NMR. We will investigate several techniques for intramolecular labeling of RNA with paramagnetic polarizing agents, including substitution of natively bound Mg2+ with Mn2+, binding of paramagnetically labeled ligands, and site-directed incorporation of paramagnetic tags based on metal chelates. This allows us to extract medium- to long-range distance constraints for multiple nuclei at once and extend the toolbox of biological NMR towards systems which cannot be effectively investigated using conventional NMR.

Team

Björn Corzilius (Principal Investigator)
 
Diane Richter