Publication – Schwalbe, Fürtig, Hengesbach

NMR structural profiling of transcriptional intermediates reveals riboswitch regulation by metastable RNA conformations.

Helmling CWacker AWolfinger MTHofacker ILHengesbach MFürtig BSchwalbe H.
J. Am. Chem. Soc., 2017 Jan 30.

Full publication here: NMR structural profiling of transcriptional intermediates reveals riboswitch regulation by metastable RNA conformations.

Abstract: 

Gene repression induced by the formation of transcriptional terminators represents a prime example for the coupling of RNA synthesis, folding, and regulation. In this context, mapping the changes in available conformational space of transcription intermediates during RNA synthesis is important to understand riboswitch function. A majority of riboswitches, an important class of small metabolite-sensing regulatory RNAs, act as transcriptional regulators, but the dependence of ligand binding and the subsequent allosteric conformational switch on mRNA transcript length has not yet been investigated. We show a strict fine-tuning of binding and sequence-dependent alterations of conformational space by structural analysis of all relevant transcription intermediates at single-nucleotide resolution for the I-A type 2′dG-sensing riboswitch from Mesoplasma florum by NMR spectroscopy. Our results provide a general framework to dissect the coupling of synthesis and folding essential for riboswitch function, revealing the importance of metastable states for RNA-based gene regulation.