Sonderforschungsbereich 902

Adenine protonation  enables c-di-GMP binding to cyclic-GAMP sensing riboswitches.

H. Keller, A. K. Weickhmann, T. Bock, J. Wöhnert.
RNA. 2018

Full publication here: Adenine protonation enables cyclic-di-GMP binding to cyclic-GAMP sensing riboswitches.

Abstract:  Read more …

An intricate balance of hydrogen bonding, ion atmosphere and
dynamics facilitates a seamless uracil to cytosine substitution in the
U-turn of the neomycin-sensing riboswitch.

M. Krepl, J. Vögele, H. Kruse, E. Duchardt-Ferner, J. Wöhnert, J.
Sponer.
Nucleic Acids Res. 2018

Full publication here: An intricate balance of hydrogen bonding, ion atmosphere and dynamics facilitates a seamless uracil to cytosine substitution in the U-turn of the neomycin-sensing riboswitch

Abstract:  Read more …

Evaluation of 15N-detected H-N correlation experiments on increasingly large RNAs.

Schnieders R, Richter C, Warhaut S, de Jesus V, Keyhani S, Duchardt-Ferner E, Keller H, Wöhnert J, Kuhn LT, Breeze AL, Bermel W, Schwalbe H, Fürtig B.
J. Biomol. NMR, 2017 Sep 6.

Full publication here: Evaluation of 15N-detected H–N correlation experiments on increasingly large RNAs

Abstract:  Read more …

Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation.

Steinert’ H, Sochor’ F, Wacker A, Buck J, Helmling C, Hiller F, Keyhani S, Noeske J, Grimm S, Rudolph MM, Keller H, Mooney RA, Landick R, Süß B, Fürtig B, Wöhnert J, Schwalbe H. eLife. 2017 May 25. Abstract Read more …

A Stably Protonated Adenine Nucleotide with a Highly Shifted pKa Value Stabilizes the Tertiary Structure of a GTP-Binding RNA Aptamer

Wolter AC, Weickhmann AK, Nasiri, AH, Hantke K, Ohlenschläger O, Wunderlich CH, Kreutz C, Duchardt-Ferner E, Wöhnert J.
Angewandte Chemie Int. Ed., 2017 Jan 2. Abstract   Read more …

An intermolecular G-quadruplex as the basis for GTP recognition in the class V-GTP aptamer.

Nasiri AH, Wurm JP, Immer C, Weickhmann AK, Wöhnert J.

RNA 11, 1750-1759. 2016 Nov 22 Abstract Read more …

What a differrence an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch.

Duchardt-Ferner E, Gottstein-Schmidtke SR, Weigand JE, Ohlenschläger O, Wurm JP, Hammann C, Suess B, Wöhnert J. Angew Chem Int Ed Engl. 2016 Jan 22. Abstract  Read more …

Backbone resonance assignments for a homolog of the essential ribosome biogenesis factor Fap7 from P. horikoshii in its nucleotide-free and -bound forms.

Hellmich UA, Wöhnert J. Biomol NMR Assign. 2013 Oct. Abstract Read more …

Essential ribosome assembly factor Fap7 regulates a hierarchy of RNA-protein interactions during small ribosomal subunit biogenesis.

Hellmich UA, Weis BL, Lioutikov A, Wurm JP, Kaiser M, Christ NA, Hantke K, Kötter P, Entian KD, Schleiff E, Wöhnert J. Proc Natl Acad Sci U S A. 2013 Sep 17. Abstract Read more …

Backbone and side chain NMR assignments for the ribosome assembly factor Nop6 from Saccharomyces cerevisiae

Wurm JP, Lioutikov A, Kötter P, Entian KD, Wöhnert J. Biomol NMR Assign. 2013 Aug 7. Abstract Read more …

Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera.

Steimer L, Wurm JP, Linden MH, Rudolph MG, Wöhnert J, Klostermeier D. Nucleic Acids Res. 2013 Jul. Abstract Read more …

Mechanisms for differentiation between cognate and near-cognate ligands by purine riboswitches.

Wacker A, Buck J, Richter C, Schwalbe H, Wöhnert J. RNA Biol, 2012 May 1. Abstract Read more …

Backbone and side chain NMR resonance assignments for an archaeal homolog of the endonuclease Nob1 involved in ribosome biogenesis.

Veith T1, Wurm JP, Duchardt-Ferner E, Weis B, Martin R, Safferthal C, Bohnsack MT, Schleiff E, Wöhnert J. Biomol NMR Assign. 2012 Apr. Abstract Read more …

RNA as drug target, in NMR of Biomolecules: Towards Mechanistic Systems Biology.

Ferner JP, Duchardt-Ferner E, Rinnenthal J, Buck J, Wöhnert J, Schwalbe H. NMR of Biomolecules: Towards Mechanistic Systems Biology, 2012 Mar. Abstract Read more …

Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs.

Wurm JP, Griese M, Bahr U, Held M, Heckel A, Karas M, Soppa J, Wöhnert J. RNA, 2012 Jan 24. Abstract Read more …

Structural and functional analysis of the archaeal endonuclease Nob1.

Veith T, Martin R, Wurm JP, Weis BL, Duchardt-Ferner E, Safferthal C, Hennig R, Mirus O, Bohnsack MT, Wöhnert J, Schleiff E. Nucleic Acids Res, 2011 Dec 10. Abstract Read more …

Influence of ground-state structure and Mg2+ binding on folding kinetics of the guanine-sensing riboswitch aptamer domain.

Buck J, Wacker A, Warkentin E, Wöhnert J, Wirmer-Bartoschek J, Schwalbe H. Nucleic Acids Res, 2011 Sept 2. Abstract Read more …

Structure and dynamics of the deoxyguanosine-sensing riboswitch studied by NMR-spectroscopy.

Wacker A, Buck J, Mathieu D, Richter C, Wöhnert J, Schwalbe H. Nucleic Acids Res, 2011 Aug. Abstract Read more …

Mechanistic insights into an engineered riboswitch: a switching element which confers riboswitch activity.

Weigand JE, Schmidtke SR, Will TJ, Duchardt-Ferner E, Hammann C, Wöhnert J, Suess B. Nucleic Acids Res, 2011. Abstract Read more …